<div dir="auto">Hi Dr. Borries, </div><div dir="auto"><br></div><div dir="auto">Thanks for your explanation. I will check with Minh or Bao for the UltraScan set up, if I needed more help, I will let you know.</div><div dir="auto"><br></div><div dir="auto">Thanks </div><div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Oct 5, 2023 at 6:14 PM Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Fatemeh,<div>AUC doesn't measure molar mass directly, it measures sedimentation (s) and diffusion coefficients (D), as well as partial concentration.</div><div><br></div><div>If the partial specific volume (PSV) is known, we can convert s and D into molar mass, but we don't know what it is. It could potentially be measured by doing a density matching experiment in different concentrations of D2O. Alternatively, we can skip this and you can make an educated guess and see what kind of distribution would be obtained with certain PSV guesses.</div><div><br></div><div>The conversion is: MW = sRT/[D(1-PSV*rho)] where R is the universal gas constant and T the temperature in kelvin. Rho is the density of the solvent (water at 20C).</div><div><br></div><div>Given that only the hyperbranched sample actually sedimented, we can proceed with that one. Also, you can use the UltraScan software to access your data in our LIMS database and simulate various scenarios of PSV and check out what molar mass distributions could be obtained with your PSV guess combined with your data. We can help you get set up with UltraScan. Also, Minh and Bao already know how to set it up.</div><div><br></div><div>Let us know how you want to proceed.</div><div><br></div><div>Regards, -Borries</div><div><br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Oct 5, 2023 at 4:13 PM Fatemeh Mehrad <<a href="mailto:mehradnia20@gmail.com" target="_blank">mehradnia20@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr"><br><div>Hi Dr. Borries, </div><div><br></div><div>Thank you so much for sending the results. Sorry for my late reply, I was on annual leave. Could you please let me know how I can calculate the Mw from these results? it is mentioned that apparently we need information to be able to calculate the average MW?</div><div><br></div><div>Thanks,</div><div>Fatemeh</div></div><img src="https://email-signature-image.com/signature.gif?u=766763&e=433243150&v=0601cd870906486b8424b4c0065d9bde651ab2332d508b48185c958e32716eea" alt="" width="0" height="0" style="width:2px;max-height:0px;overflow:hidden"><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Sep 28, 2023 at 12:43 PM Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr"><div>Hi Fatemeh and Walid,</div><div>attached is the report I briefly discussed with Walid today. Sophia ran all of the samples and was able to get a very good analysis done on the HP4 sample. it looks like only the HP4 sample actually sedimented. It looks quite heterogeneous, a small amount of the sample appears to be quite large, up to 100 S. </div><div>Please let us know if you have any questions that you would like to discuss via Zoom. I have uploaded the results to the "Supplemental Files" folder on your LIMS account.</div><div><br></div><div>Regards, -Borries</div><div><br></div><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div><div dir="ltr">
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