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Le, Bao has shared a OneDrive for Business file with you. To view it, click the link below.</p>
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<a href="https://umconnectumt-my.sharepoint.com/personal/bao_le_umt_edu/Documents/Documents/COVID/COVID%20charged%20liposomes%20paper/OD%20Spike%20and%20liposome%2002-%20Demeler's%20lab.xlsx?web=1" target="_blank" style="text-decoration: none; margin: 0px; font-size: 14px; font-family: 'Segoe UI', 'Segoe WP', 'Segoe UI WPC', Tahoma, Arial, sans-serif; color: rgb(0, 114, 198);">OD
Spike and liposome 02- Demeler's lab.xlsx</a></div>
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<p class="MsoNormal">Hi Borries and Sophia,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Please find attached the excel file with Absorbance information of Spike protein and liposome (1057-014-02) at different dilution ratios and wavelengths. It seems that we need a decent concentration (~ 0.25 mg/mL, 8x dilution from the stock)
of Spike to get a high enough OD at 280 nm. Liposome 02 seems to absorb well at 215-225 nm, I think we can dilute it 10x. I think that other liposomes would behave similar to liposome 02.<o:p></o:p></p>
<p class="MsoNormal">Do you think we can somehow perform all the measurements with the amount of Spike you’re having in hands?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Have a great weekend!<o:p></o:p></p>
<p class="MsoNormal">Bao<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Siram, Karthik <karthik.siram@mso.umt.edu> <br>
<b>Sent:</b> Friday, October 13, 2023 12:20 PM<br>
<b>To:</b> Martin, Reece <reece.martin@uleth.ca><br>
<b>Cc:</b> demelerlab@biophysics.uleth.ca; Le, Minh <Minh.Le@mso.umt.edu>; Le, Bao <bao.le@mso.umt.edu>; Borries Demeler <demeler@gmail.com><br>
<b>Subject:</b> RE: CTM LNP AUC collaboration<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif">Thank you very much, Reece! I will check them. I appreciate your efforts and time towards our project. Have a great weekend!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Times New Roman",serif">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif">Karthik<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b>From:</b> Martin, Reece <<a href="mailto:reece.martin@uleth.ca">reece.martin@uleth.ca</a>>
<br>
<b>Sent:</b> Thursday, October 12, 2023 3:33 PM<br>
<b>To:</b> Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu">karthik.siram@mso.umt.edu</a>><br>
<b>Cc:</b> <a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu">Minh.Le@mso.umt.edu</a>>; Le, Bao <<a href="mailto:bao.le@mso.umt.edu">bao.le@mso.umt.edu</a>>; Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>><br>
<b>Subject:</b> Re: CTM LNP AUC collaboration<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p style="background:white"><span style="font-size:12.0pt;color:black">Hi <span class="marknux4lgccf"><span style="background:#FFF100">Karthik</span></span>,</span><span style="color:black"><o:p></o:p></span></p>
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<p style="background:white"><span style="font-size:12.0pt;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
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<p class="elementtoproof" style="background:white"><span style="font-size:12.0pt;color:black">Our report for the measurement of the refractive index of INI-4001 and INI-2002-containing emulsion formulation 1027-123 has been uploaded to the Supporting Files
section of the project in your USLIMS account. Please let us know if you have any questions.</span><span style="color:black"><o:p></o:p></span></p>
</div>
<div>
<p style="background:white"><span style="font-size:12.0pt;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
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<p style="background:white"><span style="font-size:12.0pt;color:black">Cheers,</span><span style="color:black"><o:p></o:p></span></p>
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<p style="background:white"><span style="font-size:12.0pt;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
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<p class="elementtoproof" style="background:white"><span style="font-size:12.0pt;color:black">Reece</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="color:black">From:</span></b><span style="color:black"> Demelerlab <<a href="mailto:demelerlab-bounces@biophysics.uleth.ca">demelerlab-bounces@biophysics.uleth.ca</a>> on behalf of Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu">karthik.siram@mso.umt.edu</a>><br>
<b>Sent:</b> 11 October 2023 8:33 AM<br>
<b>To:</b> Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>><br>
<b>Cc:</b> <a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu">Minh.Le@mso.umt.edu</a>>; Le,
Bao <<a href="mailto:bao.le@mso.umt.edu">bao.le@mso.umt.edu</a>><br>
<b>Subject:</b> Re: [Demelerlab] CTM LNP AUC collaboration</span> <o:p></o:p></p>
<div>
<p class="MsoNormal"> <o:p></o:p></p>
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<div style="border:solid #646464 1.0pt;padding:2.0pt 2.0pt 2.0pt 2.0pt">
<p class="xmsonormal" style="margin:3.75pt;line-height:12.0pt;background:#FFEB9C">
<span style="font-size:10.0pt;color:black">Caution: This email was sent from someone
<b>outside of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to
<a href="mailto:phishing@uleth.ca">phishing@uleth.ca</a>.</span><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Thank you very much, Borries!</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">I am sorry for not entering the project under the AUCCanada database.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Regards,</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Karthik</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
<p class="xmsonormal"><b>From:</b> Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>>
<br>
<b>Sent:</b> Wednesday, October 11, 2023 8:03 AM<br>
<b>To:</b> Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu">karthik.siram@mso.umt.edu</a>><br>
<b>Cc:</b> Le, Bao <<a href="mailto:bao.le@mso.umt.edu">bao.le@mso.umt.edu</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu">Minh.Le@mso.umt.edu</a>>;
<a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a><br>
<b>Subject:</b> Re: CTM LNP AUC collaboration<o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Sure, no problem.<o:p></o:p></p>
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<p class="xmsonormal">Whoever is performing this experiment, can you please add this information to Karthik's project? Please note the project was not entered in the AUCCanada database, but in the CCH database.<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Finally, please upload the results in the "Supporting Files" for Karthik's account when done since this is not automatically populated through the UltraScan application.<o:p></o:p></p>
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<p class="xmsonormal">Thanks, -Borries<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">On Wed, Oct 11, 2023 at 7:56 AM Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu">karthik.siram@mso.umt.edu</a>> wrote:<o:p></o:p></p>
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<p class="xmsonormal">Good morning Borries,<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">I'm primarily interested in the refractive index of this emulsion at 20X dilution. Can you please measure refractive index of stock emulsion, 10X dil., 20X dil., 40X dil., 50X dil., 60X dil., 80X. dil., and 100 X dil.?<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Thanks, <o:p></o:p></p>
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<p class="xmsonormal">Karthik<o:p></o:p></p>
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<p class="xmsonormal"><strong><span style="font-family:"Calibri",sans-serif">From:</span></strong> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<strong><span style="font-family:"Calibri",sans-serif">Sent:</span></strong> Tuesday, October 10, 2023 4:15:08 PM<br>
<strong><span style="font-family:"Calibri",sans-serif">To:</span></strong> Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu" target="_blank">karthik.siram@mso.umt.edu</a>><br>
<strong><span style="font-family:"Calibri",sans-serif">Cc:</span></strong> Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu" target="_blank">Minh.Le@mso.umt.edu</a>>;
<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a>><br>
<strong><span style="font-family:"Calibri",sans-serif">Subject:</span></strong> Re: CTM LNP AUC collaboration<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Thanks so much, Karthik! I have seen your project request, and was just wondering if you had a particular concentration range in mind for us to test, or do you just want us to measure step-wise dilutions from the stock concentration on
down? <o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Thanks, -Borries<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">On Mon, Oct 9, 2023 at 9:24 AM Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu" target="_blank">karthik.siram@mso.umt.edu</a>> wrote:<o:p></o:p></p>
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<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Hi Borries,</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Thank you for the reminder. I will do it today.</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
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<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Regards,</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif">Karthik</span><o:p></o:p></p>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
</div>
<p class="xmsonormal"><span style="font-family:"Times New Roman",serif"> </span><o:p></o:p></p>
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<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="xmsonormal"><b>From:</b> Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>>
<br>
<b>Sent:</b> Monday, October 9, 2023 9:22 AM<br>
<b>To:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Cc:</b> Bird, Sophia <<a href="mailto:sophia.bird@uleth.ca" target="_blank">sophia.bird@uleth.ca</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu" target="_blank">Minh.Le@mso.umt.edu</a>>; Siram, Karthik <<a href="mailto:karthik.siram@mso.umt.edu" target="_blank">karthik.siram@mso.umt.edu</a>><br>
<b>Subject:</b> RE: CTM LNP AUC collaboration<o:p></o:p></p>
</div>
</div>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Thank you, Borries! I will finish that today.<o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Best,<o:p></o:p></p>
<p class="xmsonormal">Bao<o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal"><b>From:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>>
<br>
<b>Sent:</b> Monday, October 9, 2023 9:19 AM<br>
<b>To:</b> Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>><br>
<b>Cc:</b> Bird, Sophia <<a href="mailto:sophia.bird@uleth.ca" target="_blank">sophia.bird@uleth.ca</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu" target="_blank">Minh.Le@mso.umt.edu</a>><br>
<b>Subject:</b> Re: CTM LNP AUC collaboration<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">Dear Bao,<o:p></o:p></p>
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<p class="xmsonormal">please remind Karthik to enter a project. We are still waiting for your project as well.<o:p></o:p></p>
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<p class="xmsonormal">Please upload those details so we have a chance to review them before our meeting.<o:p></o:p></p>
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<p class="xmsonormal">Thanks! -Borries<o:p></o:p></p>
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<p class="xmsonormal"> <o:p></o:p></p>
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<p class="xmsonormal">On Mon, Oct 9, 2023 at 8:54 AM Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>> wrote:<o:p></o:p></p>
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<p class="xmsonormal">Thank you, Borries and Sophia!<o:p></o:p></p>
<p class="xmsonormal">A Zoom Meeting will be helpful. Minh and I will be available on Wednesday 11 Oct 4-5pm MDT, Thursday 12 Oct 1-4pm MDT, or Friday 13 Oct 11am -3pm MDT.<o:p></o:p></p>
<p class="xmsonormal">Please let me know a time that works for you, and I will send the Zoom invitation to you.<o:p></o:p></p>
<p class="xmsonormal"> <o:p></o:p></p>
<p class="xmsonormal">Best,<o:p></o:p></p>
<p class="xmsonormal">Bao<o:p></o:p></p>
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<p class="xmsonormal"><b>From:</b> Bird, Sophia <<a href="mailto:sophia.bird@uleth.ca" target="_blank">sophia.bird@uleth.ca</a>>
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<b>Sent:</b> Saturday, October 7, 2023 3:27 PM<br>
<b>To:</b> Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>>; Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu" target="_blank">Minh.Le@mso.umt.edu</a>><br>
<b>Cc:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Subject:</b> Re: CTM LNP AUC collaboration<o:p></o:p></p>
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<p class="xmsonormal"><span style="font-size:12.0pt;font-family:"Aptos",sans-serif;color:black">Hello all,</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="font-size:12.0pt;font-family:"Aptos",sans-serif;color:black">I am a lab technician in the Demeler lab and will be helping with the upcoming LNP project. T<span style="background:white">o proceed, a project submission on one
account will allow us to begin our experimental design. It would also be helpful if we could meet on Zoom sometime this week after the project is submitted to discuss our plan.</span></span><o:p></o:p></p>
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<p class="xmsonormal"><span style="font-size:12.0pt;font-family:"Aptos",sans-serif;color:black;background:white">Let us know if you have any questions regarding the project submission and a potential meeting time.</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="font-size:12.0pt;font-family:"Aptos",sans-serif;color:black;background:white">Thanks,</span><o:p></o:p></p>
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<p class="xmsonormal"><span style="font-size:12.0pt;font-family:"Aptos",sans-serif;color:black;background:white">Sophia Bird </span><o:p></o:p></p>
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