(50 pts) For this homework, please import the DMGA dataset in your imports folder into your UltraScan acccount. Make a solution using this buffer:

Sodium Chloride 300 mM
Sodium phosphate, di-basic 17 mM
Sodium phosphate, mono-basic 3 mM

For the analyte, please use this protein sequence:

0001 mgsshhhhhh ssglvprgsh meqllegkda qaihnwlsef qlegytahfl qagydvptis
0061 rmtpedltai gvtkpghrkk iaseiaqlsi aewlpsyipt dllewlcalg lpqyhkqlvs
0121 sgydsmglva dltweelqei gvnklghqkk lmlgvkrlae lrrgllqgea lse

Use any AN60Ti rotor.

Once the data are uploaded to your database, edit and analyze it with the standard workflow. A setting of s=1-5 and f/f0=1-4 should work fine. Once you completed the 2DSA-IT stage, you can proceed with the DMGA method. First, define a base model, load the analyte from above as monomer and dimer into the component list, update the concentration for the monomer only, and adjust the f/f0 value according to the pseudo-3D plot for the 2DSA-IT model. Follow along this video to perform the DMGA analysis.