[Demelerlab] Update from the Barron lab

Borries Demeler demeler at gmail.com
Tue Oct 31 06:10:52 MDT 2023


Hi Josefine,
I am glad to hear you guys figured out a way to prepare stable and
colloidal systems of abeta and LL37,
I would be happy to help you with AUC to measure the LL37/abeta complex.
Does ammonium hydroxide absorb in the low UV? If not, we can stick to low
concentrations at low UV wavelengths and measure at low concentration. With
a detailed dissolving protocol sending the powder should work fine. It may
be a while before we can get to it since one of our machines is down right
now. I would recommend sending the material to Missoula, since it is stable
(it should be fine at 4C, correct?). Then I could pick it up the next time
I am down here (driving back to Lethbridge today).

Regarding the UltraScan access, you can either install UltraScan on your
system (https://www.ultrascan3.aucsolutions.com/download.php) and then
connect it to your account in the CCH database ("Database login info" once
you are logged in), or via VNC directly on our server. I could help you
with either approach over Zoom if needed. It is possible we will have to
recreate your account because inactive accounts get removed after a while.
I checked, and your LIMS account with all of your data is still active and
ready to go.

Let me know how you want to proceed.

Regards, -Borries
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