[Myotox] SDS study of myotoxin-II

[Borries Demeler]

Dear Ana and Bruno,
We are gearing up to start the AUC experiment in the presence of SDS.
Bruno, could you please go into your LIMS account at:

	https://uslims.uleth.ca/uslims3_CCH/login.php

and update your LIMS project ("Myotoxin-II at high concentration") and 
explain in the "Buffer Components" and "Experimental Design" section 
how we should prepare the buffer and protein, i.e., what SDS
concentrations to use, what buffer to use, what protein concentrations
to try. My suggestion would be to use a moderate concentration (say, 0.5
OD 280 nm), and perhaps do a SDS titration, but want to defer to you
or Ana in case you have a better idea.

Amy is planning the experiments for the end of this week and it would be 
good to get this info soon.

On another topics:
Paul and TOny: We have sufficient new material on hand to run the
baseline NMR experiments on Myotoxin now. Please send Amy instructions
for what concentration, buffer etc. you will need and we will bring it
to you. WHen can we run the NMR experiments?

Thanks, -Borries