[Myotox] SDS study of myotoxin-II

[Bruno Lomonte]

Hi all,

fully agree with Ana G!

Bruno


+++


On 12/2/2020 20:08, Ana Gisele da Costa Neves Ferreira wrote:
> Dear Borries,
> As Amy suggested before, I think the first ideia would be to use the 
> same experimental conditions assayed by Matsui (attached paper). For 
> SV-AUC experiments, the toxin was dissolved in 20 mM Tris-HCl pH 8.0 
> and 200 mM NaCl at a concentration of 1 mg/mL in the presence or 
> absence of 1% (w/v) SDS. SDS titration seems to be a good idea too.
> Regarding NMR, what kind of experiment are you planning ?
> Regards,
> AnaG
>
> Em qua., 12 de fev. de 2020 às 18:02, Borries Demeler 
> <demeler at gmail.com <mailto:demeler at gmail.com>> escreveu:
>
>     Dear Ana and Bruno,
>     We are gearing up to start the AUC experiment in the presence of SDS.
>     Bruno, could you please go into your LIMS account at:
>
>     https://uslims.uleth.ca/uslims3_CCH/login.php
>
>     and update your LIMS project ("Myotoxin-II at high concentration")
>     and
>     explain in the "Buffer Components" and "Experimental Design" section
>     how we should prepare the buffer and protein, i.e., what SDS
>     concentrations to use, what buffer to use, what protein concentrations
>     to try. My suggestion would be to use a moderate concentration
>     (say, 0.5
>     OD 280 nm), and perhaps do a SDS titration, but want to defer to you
>     or Ana in case you have a better idea.
>
>     Amy is planning the experiments for the end of this week and it
>     would be
>     good to get this info soon.
>
>     On another topics:
>     Paul and TOny: We have sufficient new material on hand to run the
>     baseline NMR experiments on Myotoxin now. Please send Amy instructions
>     for what concentration, buffer etc. you will need and we will bring it
>     to you. WHen can we run the NMR experiments?
>
>     Thanks, -Borries
>     _______________________________________________
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>
>
>
> -- 
> Ana Gisele da C. Neves Ferreira
> Pesquisadora Titular em Saúde Pública
> Chefe do Laboratorio de Toxinologia
> Instituto Oswaldo Cruz - Fiocruz
> Pavilhao Ozorio de Almeida, sala 05
> Av. Brasil, 4365 - Manguinhos
> Rio de Janeiro - RJ
> 21040-900 Brasil
> (+5521) 2562-1381 / 98801-5726
>
>
>
>
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> http://demeler7.uleth.ca/mailman/listinfo/myotox

-- 
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Universidad de Costa Rica
San José, 11501
COSTA RICA

bruno.lomonte at ucr.ac.cr
tel. +506  2511 7888
cel. +506  8392 0012

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