[Myotox] lyophylization salts?

[Borries Demeler]

I am forwarding a message from Bruno regarding the preparation of the 
recent lyophilized myotoxin-II samples we received at ULeth:
If you don't want any salts for your NMR work we can do that with the
fresh sample we received. You could reconstitute them directly in 
straight D2O and avoid any salt in the solution. But that wouldn't be
buffered and it wouldn't be very physiological.

-b.


----- Forwarded message from Bruno Lomonte <bruno.lomonte at ucr.ac.cr> -----

Date: Thu, 20 Feb 2020 19:31:19 -0600
From: Bruno Lomonte <bruno.lomonte at ucr.ac.cr>
To: Borries Demeler <demeler at gmail.com>
Subject: Re: lyophylization salts?
User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 Thunderbird/68.4.2

Dear Borries,

sorry for the delay - busy day!  :)

no worries about salts, the protein was polished by RP-HPLC with a
water-acetonitrile gradient (+0.1% trifluoroacetic acid), so everything is
volatile and therefore no salts are expected to be present

about choosing a buffer, the protein is quite soluble in all aqueous systems,
so you have a wide range of options - I normally work with PBS when doing
bioassays (cell culture, or mouse exps) but you may choose the preferred
buffer for your methods - I would not expect any problems

please let me know if I can provide any further info

and good luck with the new experiments, can't wait to know the results!

Bruno


++++


On 20/2/2020 13:22, Borries Demeler wrote:
> Bruno,
> I am sitting here discussing with Tony and Michael the buffer
> conditions. We have lyophilized sample from you, but we don't know
> what the the salts are that were in the buffer and got co-precipitated.
> If we were to reconstitute in 1 ml of water, what kind of buffer would
> the sample be in?
> 
> Thanks for providing these details!
> 
> -Borries

-- 
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Universidad de Costa Rica
San José, 11501
COSTA RICA

bruno.lomonte at ucr.ac.cr
tel. +506  2511 7888
cel. +506  8392 0012


----- End forwarded message -----