[Myotox] lyophylization salts?

[Borries Demeler]

Agreed! I just wanted to point out that the option of running salt free,
if needed, is available. In the end I would prefer if all of our results
could be obtained under physiological conditions using PBS.

-Borries

On Fri, Feb 21, 2020 at 02:40:31PM +0000, Montina, Tony wrote:
> Hi Borries,
> I think we should just proceed with the plan we made yesterday.
> This is to first run the mycotoxin sample that Amy already provided in PBS buffer prepared using D2O.
> Cheers
> Tony
> 
> Tony Montina
> Interim Director, Science Commons Academic Operations
> Director, Magnetic Resonance Facility
> Instructor, Department of Chemistry and Biochemistry
> The University of Lethbridge, 
> 4401 University Drive West
> Lethbridge, Alberta, Canada, T1K 3M4
> Office: SA6214 Science Commons 1-403-394-3927
> Lab: SA6216 Science Commons 1-403-329-2230
> 
> -----Original Message-----
> From: Myotox <myotox-bounces at biophysics.uleth.ca> On Behalf Of Borries Demeler
> Sent: February 20, 2020 8:45 PM
> To: myotox at biophysics.uleth.ca
> Subject: Re: [Myotox] lyophylization salts?
> 
> 
> I am forwarding a message from Bruno regarding the preparation of the recent lyophilized myotoxin-II samples we received at ULeth:
> If you don't want any salts for your NMR work we can do that with the fresh sample we received. You could reconstitute them directly in straight D2O and avoid any salt in the solution. But that wouldn't be buffered and it wouldn't be very physiological.
> 
> -b.
> 
> 
> ----- Forwarded message from Bruno Lomonte <bruno.lomonte at ucr.ac.cr> -----
> 
> Date: Thu, 20 Feb 2020 19:31:19 -0600
> From: Bruno Lomonte <bruno.lomonte at ucr.ac.cr>
> To: Borries Demeler <demeler at gmail.com>
> Subject: Re: lyophylization salts?
> User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 Thunderbird/68.4.2
> 
> Dear Borries,
> 
> sorry for the delay - busy day!  :)
> 
> no worries about salts, the protein was polished by RP-HPLC with a water-acetonitrile gradient (+0.1% trifluoroacetic acid), so everything is volatile and therefore no salts are expected to be present
> 
> about choosing a buffer, the protein is quite soluble in all aqueous systems, so you have a wide range of options - I normally work with PBS when doing bioassays (cell culture, or mouse exps) but you may choose the preferred buffer for your methods - I would not expect any problems
> 
> please let me know if I can provide any further info
> 
> and good luck with the new experiments, can't wait to know the results!
> 
> Bruno
> 
> 
> ++++
> 
> 
> On 20/2/2020 13:22, Borries Demeler wrote:
> > Bruno,
> > I am sitting here discussing with Tony and Michael the buffer 
> > conditions. We have lyophilized sample from you, but we don't know 
> > what the the salts are that were in the buffer and got co-precipitated.
> > If we were to reconstitute in 1 ml of water, what kind of buffer would 
> > the sample be in?
> > 
> > Thanks for providing these details!
> > 
> > -Borries
> 
> --
> Bruno Lomonte, Ph.D.
> Instituto Clodomiro Picado
> Universidad de Costa Rica
> San José, 11501
> COSTA RICA
> 
> bruno.lomonte at ucr.ac.cr
> tel. +506  2511 7888
> cel. +506  8392 0012
> 
> 
> ----- End forwarded message -----
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