[Myotox] myotoxin2-SDS titration

[Borries Demeler]

The s-value is reflective of the size of the molecule. A 5.5 s species is
much bigger than a 2 s species. The y axis tells you the relative amount of
species with a given s-value. It is a standard integral sedimentation
distribution. I am happy to have a zoom meeting to discuss further these
interesting trends.
-Borries

On Thu, Apr 1, 2021 at 4:20 PM Hazendonk, Paul <paul.hazendonk at uleth.ca>
wrote:

> What is the best way to interpret these curves.  I have not seen
> ultracentrifugation data since 1991.  Should we be looking at the position
> of the inflection point?
>
>
> -----Original Message-----
> From: Myotox <myotox-bounces at biophysics.uleth.ca> On Behalf Of Borries
> Demeler
> Sent: April 1, 2021 11:57 AM
> To: Myotoxin-II discussion <myotox at biophysics.uleth.ca>
> Subject: Re: [Myotox] myotoxin2-SDS titration
>
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>
> Dear all,
> we have results back from the myotoxin-2 SDS titration experiments in AUC.
> I feel these experiments provided some very interesting results and are
> probably the most informative ones we have done so far. I also think that
> they will be extremely helpful in designing the *correct* NMR experiment. I
> went through the effort to write this up with figures, please review
> carefully the attached document and read the explanations I believe explain
> these results (which were quite unintuitive at first, but now make sense to
> me). Then let's schedule another meeting and discussion of next steps.
>
> -Borries
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