[Myotox] myotoxin2-SDS titration

[Hazendonk, Paul]

So if you have association-dissociation taking place, the slope at the inflection point does not change with speed.  I guess the exchange rates are too high.  How about for very slow processes?  I guess at that point you would see separate inflections.

-----Original Message-----
From: Myotox <myotox-bounces at biophysics.uleth.ca> On Behalf Of Borries Demeler
Sent: April 1, 2021 5:42 PM
To: Myotoxin-II discussion <myotox at biophysics.uleth.ca>
Subject: Re: [Myotox] myotoxin2-SDS titration

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Varying the speed only affects the resolution:

high speed: good resolution on s, poor resolution on D low speed: good resolution on D, poor resolution on s.

-Borries

On Thu, Apr 01, 2021 at 11:16:34PM +0000, Paul Hazendonk wrote:
> Can you do dynamic studies by varying the speed of centrifugation?  I.e. the slope as a function of speed?
>
> From: Myotox <myotox-bounces at biophysics.uleth.ca> On Behalf Of Borries 
> Demeler
> Sent: April 1, 2021 4:37 PM
> To: Myotoxin-II discussion <myotox at biophysics.uleth.ca>
> Subject: Re: [Myotox] myotoxin2-SDS titration
>
> Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to phishing at uleth.ca<mailto:phishing at uleth.ca>.
>
> The s-value is reflective of the size of the molecule. A 5.5 s species is much bigger than a 2 s species. The y axis tells you the relative amount of species with a given s-value. It is a standard integral sedimentation distribution. I am happy to have a zoom meeting to discuss further these interesting trends.
> -Borries
>
> On Thu, Apr 1, 2021 at 4:20 PM Hazendonk, Paul <paul.hazendonk at uleth.ca<mailto:paul.hazendonk at uleth.ca>> wrote:
> What is the best way to interpret these curves.  I have not seen ultracentrifugation data since 1991.  Should we be looking at the position of the inflection point?
>
>
> -----Original Message-----
> From: Myotox 
> <myotox-bounces at biophysics.uleth.ca<mailto:myotox-bounces at biophysics.u
> leth.ca>> On Behalf Of Borries Demeler
> Sent: April 1, 2021 11:57 AM
> To: Myotoxin-II discussion 
> <myotox at biophysics.uleth.ca<mailto:myotox at biophysics.uleth.ca>>
> Subject: Re: [Myotox] myotoxin2-SDS titration
>
> Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to phishing at uleth.ca<mailto:phishing at uleth.ca>.
>
>
> Dear all,
> we have results back from the myotoxin-2 SDS titration experiments in AUC. I feel these experiments provided some very interesting results and are probably the most informative ones we have done so far. I also think that they will be extremely helpful in designing the *correct* NMR experiment. I went through the effort to write this up with figures, please review carefully the attached document and read the explanations I believe explain these results (which were quite unintuitive at first, but now make sense to me). Then let's schedule another meeting and discussion of next steps.
>
> -Borries
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