[Myotox] myotoxin2-SDS titration

[Bruno Lomonte]

Dear all,

I was refreshing my memory on some older studies on a myotoxin that is 
highly similar to Mt-II, and for which a paper on its interaction with 
SDS had been presented in 2007 using circular dichroism, here attached. 
Owing to my *very *limited understanding of biophysical experiments, I 
do not know if this article could provide us (or not) with any useful 
insights to the discussions and interpretations of current experiments.

The only other study on the SDS interaction is more recent (2019), by a 
Japanese group, but I think we had already shared this few months ago 
and actually sparked the initiatives to perform the SDS experiments. 
Here I attach it in case some of us in the list did not see it before.

Looking forward to the discussion tomorrow

Best regards to all

Bruno


PS: apologies for the personal yellow highlights in the files... I guess 
they are all deletable

+++++++++++++++




On 4/1/2021 11:56 AM, Borries Demeler wrote:
> Dear all,
> we have results back from the myotoxin-2 SDS titration experiments in
> AUC. I feel these experiments provided some very interesting results and
> are probably the most informative ones we have done so far. I also think
> that they will be extremely helpful in designing the *correct* NMR
> experiment. I went through the effort to write this up with figures,
> please review carefully the attached document and read the explanations
> I believe explain these results (which were quite unintuitive at first,
> but now make sense to me). Then let's schedule another meeting and
> discussion of next steps.
>
> -Borries
>
> _______________________________________________
> Myotox mailing list
> Myotox at biophysics.uleth.ca
> https://demeler7.uleth.ca/mailman/listinfo/myotox

-- 
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Facultad de Microbiología
Universidad de Costa Rica
San José, COSTA RICA

tel.of. (+506) 2511 7888
bruno.lomonte at ucr.ac.cr

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