[Myotox] Myotoxin-II sequence

Borries Demeler demeler at gmail.com
Mon Sep 5 14:44:32 MDT 2022


Hi everyone,
I'm currently writing up the AUC results, but come across an inconsistency I m hoping someone can clear up for me.
In the literature, I see myotoxin-II from Bothrops asper referred to as the class-2 myotoxin Lys-49 phospholipases.
When I look at the protein sequence (which I believe I obtained from Bruno), I see Lys (K) at position 48, not 49:

0001 slfelgkmil qetgknpaks ygaygcncgv lgrgkpkdat drccyvhkcc ykkltgcnpk
0061 kdrysyswkd ktivcgenns clkelcecdk avaiclrenl ntynkkyryy lkplckkada
0121 c 

Am I missing a residue? I found the exact same sequence in the protein database.

Since we determined very precise sed. and diff. coefficients, I could use the precisely known molar mass from sequence
to calculate an accurate partial specific volume and anisotropy. Before I use this sequence, it would be good if someone
could confirm it. Ana, what do you get from mass spec? Do you have some mass spec data we can include in this manuscript?

Thanks, -Borries


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