[Demelerlab] Fatemeh's report draft

[Fatemeh Mehrad]

Hi Dr. Borries,

Thanks for your explanation. I will check with Minh or Bao for the
UltraScan set up, if I needed more help, I will let you know.

Thanks

On Thu, Oct 5, 2023 at 6:14 PM Borries Demeler <demeler at gmail.com> wrote:

> Fatemeh,
> AUC doesn't measure molar mass directly, it measures sedimentation (s) and
> diffusion coefficients (D), as well as partial concentration.
>
> If the partial specific volume (PSV) is known, we can convert s and D into
> molar mass, but we don't know what it is. It could potentially be measured
> by doing a density matching experiment in different concentrations of D2O.
> Alternatively, we can skip this and you can make an educated guess and see
> what kind of distribution would be obtained with certain PSV guesses.
>
> The conversion is: MW = sRT/[D(1-PSV*rho)] where R is the universal gas
> constant and T the temperature in kelvin. Rho is the density of the solvent
> (water at 20C).
>
> Given that only the hyperbranched sample actually sedimented, we can
> proceed with that one. Also, you can use the UltraScan software to access
> your data in our LIMS database and simulate various scenarios of PSV and
> check out what molar mass distributions could be obtained with your PSV
> guess combined with your data. We can help you get set up with UltraScan.
> Also, Minh and Bao already know how to set it up.
>
> Let us know how you want to proceed.
>
> Regards, -Borries
>
>
>
> On Thu, Oct 5, 2023 at 4:13 PM Fatemeh Mehrad <mehradnia20 at gmail.com>
> wrote:
>
>>
>> Hi Dr. Borries,
>>
>> Thank you so much for sending the results. Sorry for my late reply, I was
>> on annual leave. Could you please let me know how I can calculate the Mw
>> from these results? it is mentioned  that apparently we need information to
>> be able to calculate the average MW?
>>
>> Thanks,
>> Fatemeh
>>
>> On Thu, Sep 28, 2023 at 12:43 PM Borries Demeler <demeler at gmail.com>
>> wrote:
>>
>>> Hi Fatemeh and Walid,
>>> attached is the report I briefly discussed with Walid today. Sophia ran
>>> all of the samples and was able to get a very good analysis done on the HP4
>>> sample. it looks like only the HP4 sample actually sedimented. It looks
>>> quite heterogeneous, a small amount of the sample appears to be quite
>>> large, up to 100 S.
>>> Please let us know if you have any questions that you would like to
>>> discuss via Zoom. I have uploaded the results to the "Supplemental Files"
>>> folder on your LIMS account.
>>>
>>> Regards, -Borries
>>>
>>>
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