[Demelerlab] Fatemeh's report draft

[Borries Demeler]

Sounds good, Fatemeh! Let me know if you need help.
We should also schedule a meeting to discuss the results from your samples.
-Borries

On Fri, Oct 6, 2023 at 12:54 PM Fatemeh Mehrad <mehradnia20 at gmail.com>
wrote:

> Hi Dr. Borries,
>
> Thanks for your explanation. I will check with Minh or Bao for the
> UltraScan set up, if I needed more help, I will let you know.
>
> Thanks
>
> On Thu, Oct 5, 2023 at 6:14 PM Borries Demeler <demeler at gmail.com> wrote:
>
>> Fatemeh,
>> AUC doesn't measure molar mass directly, it measures sedimentation (s)
>> and diffusion coefficients (D), as well as partial concentration.
>>
>> If the partial specific volume (PSV) is known, we can convert s and D
>> into molar mass, but we don't know what it is. It could potentially be
>> measured by doing a density matching experiment in different concentrations
>> of D2O. Alternatively, we can skip this and you can make an educated guess
>> and see what kind of distribution would be obtained with certain PSV
>> guesses.
>>
>> The conversion is: MW = sRT/[D(1-PSV*rho)] where R is the universal gas
>> constant and T the temperature in kelvin. Rho is the density of the solvent
>> (water at 20C).
>>
>> Given that only the hyperbranched sample actually sedimented, we can
>> proceed with that one. Also, you can use the UltraScan software to access
>> your data in our LIMS database and simulate various scenarios of PSV and
>> check out what molar mass distributions could be obtained with your PSV
>> guess combined with your data. We can help you get set up with UltraScan.
>> Also, Minh and Bao already know how to set it up.
>>
>> Let us know how you want to proceed.
>>
>> Regards, -Borries
>>
>>
>>
>> On Thu, Oct 5, 2023 at 4:13 PM Fatemeh Mehrad <mehradnia20 at gmail.com>
>> wrote:
>>
>>>
>>> Hi Dr. Borries,
>>>
>>> Thank you so much for sending the results. Sorry for my late reply, I
>>> was on annual leave. Could you please let me know how I can calculate the
>>> Mw from these results? it is mentioned  that apparently we need information
>>> to be able to calculate the average MW?
>>>
>>> Thanks,
>>> Fatemeh
>>>
>>> On Thu, Sep 28, 2023 at 12:43 PM Borries Demeler <demeler at gmail.com>
>>> wrote:
>>>
>>>> Hi Fatemeh and Walid,
>>>> attached is the report I briefly discussed with Walid today. Sophia ran
>>>> all of the samples and was able to get a very good analysis done on the HP4
>>>> sample. it looks like only the HP4 sample actually sedimented. It looks
>>>> quite heterogeneous, a small amount of the sample appears to be quite
>>>> large, up to 100 S.
>>>> Please let us know if you have any questions that you would like to
>>>> discuss via Zoom. I have uploaded the results to the "Supplemental Files"
>>>> folder on your LIMS account.
>>>>
>>>> Regards, -Borries
>>>>
>>>>
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