[Demelerlab] Fatemeh's report draft

[Fatemeh Mehrad]

Thanks, I will let you know.

On Fri, Oct 6, 2023 at 12:56 PM Borries Demeler <demeler at gmail.com> wrote:

> Sounds good, Fatemeh! Let me know if you need help.
> We should also schedule a meeting to discuss the results from your samples.
> -Borries
>
> On Fri, Oct 6, 2023 at 12:54 PM Fatemeh Mehrad <mehradnia20 at gmail.com>
> wrote:
>
>> Hi Dr. Borries,
>>
>> Thanks for your explanation. I will check with Minh or Bao for the
>> UltraScan set up, if I needed more help, I will let you know.
>>
>> Thanks
>>
>> On Thu, Oct 5, 2023 at 6:14 PM Borries Demeler <demeler at gmail.com> wrote:
>>
>>> Fatemeh,
>>> AUC doesn't measure molar mass directly, it measures sedimentation (s)
>>> and diffusion coefficients (D), as well as partial concentration.
>>>
>>> If the partial specific volume (PSV) is known, we can convert s and D
>>> into molar mass, but we don't know what it is. It could potentially be
>>> measured by doing a density matching experiment in different concentrations
>>> of D2O. Alternatively, we can skip this and you can make an educated guess
>>> and see what kind of distribution would be obtained with certain PSV
>>> guesses.
>>>
>>> The conversion is: MW = sRT/[D(1-PSV*rho)] where R is the universal gas
>>> constant and T the temperature in kelvin. Rho is the density of the solvent
>>> (water at 20C).
>>>
>>> Given that only the hyperbranched sample actually sedimented, we can
>>> proceed with that one. Also, you can use the UltraScan software to access
>>> your data in our LIMS database and simulate various scenarios of PSV and
>>> check out what molar mass distributions could be obtained with your PSV
>>> guess combined with your data. We can help you get set up with UltraScan.
>>> Also, Minh and Bao already know how to set it up.
>>>
>>> Let us know how you want to proceed.
>>>
>>> Regards, -Borries
>>>
>>>
>>>
>>> On Thu, Oct 5, 2023 at 4:13 PM Fatemeh Mehrad <mehradnia20 at gmail.com>
>>> wrote:
>>>
>>>>
>>>> Hi Dr. Borries,
>>>>
>>>> Thank you so much for sending the results. Sorry for my late reply, I
>>>> was on annual leave. Could you please let me know how I can calculate the
>>>> Mw from these results? it is mentioned  that apparently we need information
>>>> to be able to calculate the average MW?
>>>>
>>>> Thanks,
>>>> Fatemeh
>>>>
>>>> On Thu, Sep 28, 2023 at 12:43 PM Borries Demeler <demeler at gmail.com>
>>>> wrote:
>>>>
>>>>> Hi Fatemeh and Walid,
>>>>> attached is the report I briefly discussed with Walid today. Sophia
>>>>> ran all of the samples and was able to get a very good analysis done on the
>>>>> HP4 sample. it looks like only the HP4 sample actually sedimented. It looks
>>>>> quite heterogeneous, a small amount of the sample appears to be quite
>>>>> large, up to 100 S.
>>>>> Please let us know if you have any questions that you would like to
>>>>> discuss via Zoom. I have uploaded the results to the "Supplemental Files"
>>>>> folder on your LIMS account.
>>>>>
>>>>> Regards, -Borries
>>>>>
>>>>>
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