[Myotox] [tony.montina at uleth.ca: RE: Myotoxin-II]

Sun Jun 7 17:17:21 MDT 2020

Hello all,

very happy to hear that the NMR experiment went OK! and agree that it 
would be nice to complement results with additional comparisons in the 
presence of SDS or other conditions

I will prepare more myotoxin-II, although it may take a bit longer than 
usual (since I have limited access to the institute due to the 
coronavirus confinement). In any case I will be in contact when ready to 
ship.

Hope you are all fine! here the number of cases was going really down - 
good work done by the goverment - , but in these last days things went 
out of hands with our neighbors in the North (Nicaragua), desperately 
crossing the border illegally (and I fully understand their situation) 
and now infections are again growing in Costa Rica:

Best wishes to all,

Bruno

+++

On 6/7/2020 3:05 PM, Henrickson, Amy wrote:
> Hello,
>
> For the NMR studies I provided about 2mg of sample therefore they 
> would like 4-6mg more.
>
> I do still have what I would guess to be about 0.5mg of lyophilized 
> sample left.
> I also saved most of the samples that I ran in the AUC however, they 
> have been sitting at room temp suspended in buffer, therefore I am not 
> sure if it would be useful or not.
>
> Cheers,
>
> Amy Henrickson
> ------------------------------------------------------------------------
> *From:* Myotox <myotox-bounces at biophysics.uleth.ca> on behalf of 
> Borries Demeler <demeler at gmail.com>
> *Sent:* Sunday, June 7, 2020 12:31 PM
> *To:* myotox at biophysics.uleth.ca <myotox at biophysics.uleth.ca>
> *Subject:* [Myotox] [tony.montina at uleth.ca: RE: Myotoxin-II]
> Caution: This email was sent from someone outside of the University of 
> Lethbridge. Do not click on links or open attachments unless you know 
> they are safe. Please forward suspicious emails to phishing at uleth.ca.
>
> Hello Bruno,
> Paul's group completed the first NMR experiment on Myotoxin-II, which
> according to Paul and Tony provided very nice signal. I thought it
> would be useful to compare the +/- SDS conditions by NMR as well and
> see if we can learn something new about the interaction of SDS with
> myotoxin-II. Everybody, please let us know if you agree with this.
>
> As you can see from the communication below, Tony would require additional
> protein to perform these studies and run the necessary controls. Amy,
> can you please advise Bruno of the amounts? It would be 2-3 times the
> amount you gave to Tony previously. Once we know the amounts, please
> let us know if it is possible to send one more batch of myotoxin-II.
>
> My guess is that it would be best if we could obtain the sample in
> lyophilized format, so it can be resuspended directly in aqueous buffer
> made with either light or heavy water. Importantly, if the protein is
> dissolved in D2O, lyophilized sample would allow us to reconstitute
> directly in D2O and avoid contamination by light water, which gives
> different background signals in NMR. Tony, please confirm if this is
> what you would like to have. THe SDS concentration should be 1%, which
> is what we measured by AUC.
>
> Paul: Can you please describe the results you obtained and let us know
> based on the preliminary data what we can expect to learn from the
> additional NMR experiments, especially with respect to protein structure
> and interaction with SDS. If this provides greater insights into
> the strange protein behavior in SDS I think it would really make our
> manuscript a lot more interesting!
>
> Thank you all! -Borries
>
>
> ----- Forwarded message from "Montina, Tony" <tony.montina at uleth.ca> -----
>
> Date: Sun, 7 Jun 2020 18:04:01 +0000
> From: "Montina, Tony" <tony.montina at uleth.ca>
> To: Borries Demeler <demeler at gmail.com>, "Hazendonk, Paul" 
> <paul.hazendonk at uleth.ca>
> CC: "Henrickson, Amy" <amy.henrickson at uleth.ca>
> Subject: RE: Myotoxin-II
>
> Hello Borries,
> I believe that Mike used most of the sample that Amy gave him to make 
> up the myotoxin in D2O and PBS.
> It would probably be worthwhile to ask Bruno to send more, as we will 
> want to prepare the NMR samples under several conditions.
> For example, we might want a sample (as you suggested) with SDS, we 
> might want another sample with H2O and D2O (for exchange purposes), etc.
> Amy would have a better idea of how much she just gave us, but I would 
> suggest two to three times that amount.
> Does that work
> Tony
>
> _______________________________________________
> Tony Montina
> Director, Science Operations
> Director, Magnetic Resonance Facility
> Instructor, Department of Chemistry and Biochemistry
> Faculty of Arts and Science
> The University of Lethbridge
> Lethbridge, Alberta, Canada, T1K 3M4
> Office: 1-403-394-3927
> Lab: 1-403-329-2230
>
> -----Original Message-----
> From: Borries Demeler <demeler at gmail.com>
> Sent: June 5, 2020 4:23 PM
> To: Hazendonk, Paul <paul.hazendonk at uleth.ca>
> Cc: Montina, Tony <tony.montina at uleth.ca>; Henrickson, Amy 
> <amy.henrickson at uleth.ca>
> Subject: Re: Myotoxin-II
>
> Caution: This email was sent from someone outside of the University of 
> Lethbridge. Do not click on links or open attachments unless you know 
> they are safe. Please forward suspicious emails to phishing at uleth.ca.
>
> Tony/Amy, Paul mentioned that we could do this experiment in the 
> presence of SDS. How much material would we need and how much do we 
> still have on hand? If we don't have enough we should contact Bruno 
> and ask him to milk some more snakes :) -b.
>
>
> On Fri, Jun 05, 2020 at 07:02:11PM +0000, Hazendonk, Paul wrote:
> > Hi Borries
> >
> > We have a 1D proton that makes sense to me now. We have some T1 
> data. I am waiting for the T2 and DOSY results. Mike could you updaye 
> us on when these will be done.
> >
> > Cheers
> >
> > Paul
> >
> > Sent from Outlook Mobile<https://aka.ms/blhgte>
> >
>
> ----- End forwarded message -----
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-- 
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Universidad de Costa Rica
San José, 11501
COSTA RICA

bruno.lomonte at ucr.ac.cr
tel. +506  2511 7888
cel. +506  8392 0012

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