[Myotox] Myotoxin-II

[Borries Demeler]

FOr me the most interesting part would be to learn the structural
arrangement of the SDS molecules around the protein, and how it changes
the protein itself, vs. how does it change the conformation of the
protein in the presence of SDS so that it goes from monomer to dimer?

THis is the central question we are trying to answer. If this is not the
type of information we can get readily, what other information can be
obtained that would give us more coarse-grained information?

Thanks, -Borries

P.S. please reply to myotox at biophysics.uleth.ca so everyone gets to see
your response. Bruno and Ana may have some additional ideas as to what 
we could get out of the NMR experiments. Please feel free to tune in...


On Mon, Jun 08, 2020 at 07:46:42PM +0000, Montina, Tony wrote:
> Hello all,
> I agree with Paul. An idea of what you are looking for in this first paper would be helpful in guiding the NMR experimental work.
> Tony
> 
> _______________________________________________
> Tony Montina
> Director, Science Operations
> Director, Magnetic Resonance Facility
> Instructor, Department of Chemistry and Biochemistry
> Faculty of Arts and Science
> The University of Lethbridge
> Lethbridge, Alberta, Canada, T1K 3M4
> Office: 1-403-394-3927
> Lab: 1-403-329-2230
> 
> From: Hazendonk, Paul <paul.hazendonk at uleth.ca>
> Sent: June 8, 2020 1:32 PM
> To: Borries Demeler <demeler at gmail.com>; Montina, Tony <tony.montina at uleth.ca>
> Cc: Henrickson, Amy <amy.henrickson at uleth.ca>
> Subject: Re: Myotoxin-II
> 
> I think we need to ask ourselves what type of structural insights are the most important for this particular story. This will help us set the goals for the level of strucural information sought. Some may be achieved with partial imformation other may require us to do a complete spectral assignment. The ammount of work required, hence time, would be dramatically different.
> Sent from Outlook Mobile<https://aka.ms/blhgte>
> 
> ________________________________
> From: Montina, Tony <tony.montina at uleth.ca<mailto:tony.montina at uleth.ca>>
> Sent: Sunday, June 7, 2020 12:04:01 PM
> To: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>; Hazendonk, Paul <paul.hazendonk at uleth.ca<mailto:paul.hazendonk at uleth.ca>>
> Cc: Henrickson, Amy <amy.henrickson at uleth.ca<mailto:amy.henrickson at uleth.ca>>
> Subject: RE: Myotoxin-II
> 
> Hello Borries,
> I believe that Mike used most of the sample that Amy gave him to make up the myotoxin in D2O and PBS.
> It would probably be worthwhile to ask Bruno to send more, as we will want to prepare the NMR samples under several conditions.
> For example, we might want a sample (as you suggested) with SDS, we might want another sample with H2O and D2O (for exchange purposes), etc.
> Amy would have a better idea of how much she just gave us, but I would suggest two to three times that amount.
> Does that work
> Tony
> 
> _______________________________________________
> Tony Montina
> Director, Science Operations
> Director, Magnetic Resonance Facility
> Instructor, Department of Chemistry and Biochemistry
> Faculty of Arts and Science
> The University of Lethbridge
> Lethbridge, Alberta, Canada, T1K 3M4
> Office: 1-403-394-3927
> Lab: 1-403-329-2230
> 
> -----Original Message-----
> From: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>
> Sent: June 5, 2020 4:23 PM
> To: Hazendonk, Paul <paul.hazendonk at uleth.ca<mailto:paul.hazendonk at uleth.ca>>
> Cc: Montina, Tony <tony.montina at uleth.ca<mailto:tony.montina at uleth.ca>>; Henrickson, Amy <amy.henrickson at uleth.ca<mailto:amy.henrickson at uleth.ca>>
> Subject: Re: Myotoxin-II
> 
> Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Please forward suspicious emails to phishing at uleth.ca<mailto:phishing at uleth.ca>.
> 
> Tony/Amy, Paul mentioned that we could do this experiment in the presence of SDS. How much material would we need and how much do we still have on hand? If we don't have enough we should contact Bruno and ask him to milk some more snakes :) -b.
> 
> 
> On Fri, Jun 05, 2020 at 07:02:11PM +0000, Hazendonk, Paul wrote:
> > Hi Borries
> >
> > We have a 1D proton that makes sense to me now. We have some T1 data. I am waiting for the T2 and DOSY results. Mike could you updaye us on when these will be done.
> >
> > Cheers
> >
> > Paul
> >
> > Sent from Outlook Mobile<https://aka.ms/blhgte>
> >