[Myotox] Myotoxin-II

[Ana Gisele da Costa Neves Ferreira]

Hi Borries and colleagues,
Thank you for your last emails and for the very interesting results. And
sorry for my late reply, things are very busy here... I´ll write a longer
email with some comments later this week.
Best regards,
AnaG

Em ter., 9 de jun. de 2020 às 23:27, Borries Demeler <demeler at gmail.com>
escreveu:

>
> Bruno and Ana,
> do you have some comments on what we should attempt here for our paper?
> In any case, let's wait until Bruno can send some more samples to ship
> here. Then we should attempt the SDS experiments and start by comparing
> differences coming from the SDS contacts with the protein. I think that
> would already be quite interesting. The protein dynamics may be
> interesting if there is reduction in dynamics upon dimerization and we
> could see that. It sounds like we can get a lot of details already
> without the full assignments. Hence, I really believe this could be
> worthwhile.
> Thanks a lot, Paul! Looking forward to the SDS data...
>
> Best wishes to all, and stay safe whereever you are!
>
> -Borries
>
> On Mon, Jun 08, 2020 at 08:40:15PM +0000, Hazendonk, Paul wrote:
> > The DOSY data of the protein and mixtures should shed some light in its
> size and degree of association with the SDS.
> >
> > Without doing a complete assignment we can probably identify what type
> AAs are involved with interactions with SDS through changes in there
> shifts.  However we cannot be unambiguous about which one in particular it
> is in the sequence.
> >
> > Relaxation  times of the H-alpha will give use some insights into
> changes in ptn dynamics. The NH signal can tell us about the motion in the
> alpha helical regions. The buried methyl signals can tell us about  the
> hydrophobic regions.
> >
> > Any ambiguities would be lifted with a full assignment, however a
> worthwhile endeavor, will take a lot of time.
> >
> > Sent from Outlook Mobile<https://aka.ms/blhgte>
> >
>
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>
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-- 
Ana Gisele da C. Neves Ferreira
Pesquisadora Titular em Saúde Pública
Chefe do Laboratorio de Toxinologia
Instituto Oswaldo Cruz - Fiocruz
Pavilhao Ozorio de Almeida, sala 05
Av. Brasil, 4365 - Manguinhos
Rio de Janeiro - RJ
21040-900 Brasil
(+5521) 2562-1381 / 98801-5726
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