[Myotox] Myotoxin-II

[Borries Demeler]

Bruno and Ana,
do you have some comments on what we should attempt here for our paper?
In any case, let's wait until Bruno can send some more samples to ship
here. Then we should attempt the SDS experiments and start by comparing 
differences coming from the SDS contacts with the protein. I think that 
would already be quite interesting. The protein dynamics may be
interesting if there is reduction in dynamics upon dimerization and we
could see that. It sounds like we can get a lot of details already
without the full assignments. Hence, I really believe this could be
worthwhile.
Thanks a lot, Paul! Looking forward to the SDS data...

Best wishes to all, and stay safe whereever you are!

-Borries

On Mon, Jun 08, 2020 at 08:40:15PM +0000, Hazendonk, Paul wrote:
> The DOSY data of the protein and mixtures should shed some light in its size and degree of association with the SDS.
> 
> Without doing a complete assignment we can probably identify what type AAs are involved with interactions with SDS through changes in there shifts.  However we cannot be unambiguous about which one in particular it is in the sequence.
> 
> Relaxation  times of the H-alpha will give use some insights into changes in ptn dynamics. The NH signal can tell us about the motion in the alpha helical regions. The buried methyl signals can tell us about  the hydrophobic regions.
> 
> Any ambiguities would be lifted with a full assignment, however a worthwhile endeavor, will take a lot of time.
> 
> Sent from Outlook Mobile<https://aka.ms/blhgte>
> 

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